DNA
Part:BBa_K891000:Design
Designed by: Ryan Muller Group: iGEM12_Arizona_State (2012-10-02)
GFPT1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The coding sequence for E.coli Keio strain BW31005 (CGSC#8185)genomic GFP was blasted sequence against the entire genome to identify a region with high probe binding specificity. The sequence needed to be absent of YCCTT and AAGGR motifs, but accidentally includes a TCCTT site in the middle of the probe.
Source
GFPT1 originates from genomic GFP in the E.coli Keio strain BW31005 (CGSC#8185).
References
"E. Coli Genetic Resources at Yale CGSC, The Coli Genetic Stock Center." Strain. N.p., n.d. Web. 03 Oct. 2012. <http://cgsc.biology.yale.edu/Strain.php?ID=89135>.